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ISTC Project No. KR1429

“Epidemiological monitoring of avian virus circulation in the nature, an estimation of biological danger of the pestholes in Kyrgyz Republic”

QuarterlyTechnical Report

on the work performed from 01.12.2010 to 28.02.2011

- Quarter no. 7 -

  State Sanitary and Epidemiological Supervision Department under the Ministry of

Health of Kyrgyz Republic

address: 535, Frunze st, Bishkek, 720033, , Kyrgyz Republic

Project Manager

Kasymbekova Kaliya Toktosunovna

Doctor of Medicine, PhD, M.D.

Signature / Signature / 20 March 2011

1.Summary of Technical Progress

1.1.Current Technical Status

Task

Start

End

Status / Comments

Task 1 Definition of migration birds concentration places:

- purchasing of the equipment, expendable materials and others

- organization of expeditions to collect field material

-observation over terms and intensity of migrations

-ringing of 900 birds

01.12.2011

28.02.2011

Record-keeping of waterfowls of Issyk-Kul lake is carried out. Materials of registration have been processed on Issyk-Kul oblast as well as materials of tagging of birds. New tools for birds catching have been repaired and created. Biological samples from 20 birds have been taken.

Due to lack of funding expeditionary trips for the collection of biological samples have not been carried out. Only short-term expedition was organized to Issyk-Kul oblast to register wintering waterfowls on Issyk-Kul lake. In the course of this expedition biological samples from 20 birds have been collected. Catching tools and expedition equipment have been repaired. 23 nets to catch birds with a total length of 250 meters have been produced. The booklet about the project and information for the annual “Innovative Technologies of National Academy of Sciences” has been prepared as well as the article for collected papers of Issyk-Kul nature reserve -“Colonies of waterfowls of Issyk-Kul lake”. Materials for tagging of birds have been processed and reports have been sent to the center of ringing in Almaty (Kazakhstan). Permits for catching and tagging of birds, to visit bordering area and other necessary documents have been obtained.

Task 2

The organization of molecular - genetic and virological researches of avian  flu

Quarter 3

Quarter 12

Work is carried out ahead of the work plan.

Due to the absence of poultry farms which can deliver sterile chicken embryos, we used cell cultures for virus isolation. Virological researches have been conducted with transplantable line of cell culture of dog’s kidney (MDCK).

Task 3

Collection and analysis of literary data on H6N1 avian influenza

01.09.2010

30.11.2010

Scheduled issues of epizootic (epidemiological) monitoring are performed.

Collection and development of materials on postmortem diagnostics of avian influenza

01.09.2010

30.11.2010

Postmortem examination of 26 dead birds of 3 species found in Issyk-Kul oblast.

Clinical epidemiological diagnostics of H6N1 avian influenza among waterfowls and inhabited near lakes and ponds birds

01.09.2010

30.11.2010

In the field conditions clinical examination of birds is not conducted.

Task 4

Molecular-genetic characteristic of avian flu virus, circulating in natural reservoirs.

Quarter 1

Quarter 12

Subtyping of viruses is conducted. We are planning to conduct strain sequencing with foreign collaborators.

Task 5

Mapping of households farms for the presence of poultry

01.09.2010

30.11.2010

Four districts of Talas oblast have been surveyed by visits for the presence of poultry.

1.2.Tasks of the work plan

Task 1: Definition of migration birds concentration places

        1. State / Situation at the beginning of the current quarter

Дописать

        1. Fulfilled work
        1. Results by the end of the current quarter

Дописать

Biological samples were taken from birds belonging to 2 species (Table 1).

Table 1

The list of birds from which samples on influenza virus were taken in Quarter 7 (December 2010- February 2011)

Bird species (English, Latin, Russian)

Number

Common Coot (Fulica atra) - лысуха

1

Whooper swan (Cygnus cygnus) - лебедь-кликун

19

Total:

20

        1. Personnel Commitments

Name

Category

Days

Ostachshenko Anatolii

I

31

Toropova Valentina

I

15

Kumushaliev Bektur

I

25

Davletbakov Askar

II

10

Milko Dmitrii

III

6

Chernova Julia

III

30

Vorobev Aleksandr

IV

28

Total

Task 2: The organization of molecular - genetic and virological researches of avian  flu

        1. State / Situation at the beginning of the current quarter
          At the project beginning molecular genetic researches of birds have been conducted. Virological researches on cell culture of dog’s kidney was conducted, however, there was no virus isolation. There were samples with cytopathogenic effects.
        1. Fulfilled work

Total number of conducted researches is 343.  33 species of birds were exposed to molecular-genetic researches, presence of influenza A were detected in 3 bird species.  

        1. Results by the end of the current quarter

Table 2

Results of monthly molecular-genetic researches of birds

#

Month

Number of researched birds

Out of them positive

A influenza positive

Н1

Н3

Н5

1

IХ-2010

194

0

 

 

 

 

2

X-2010

93

2

2

0

0

0

3

XI-2010

20

0

0

0

0

0

 

Total

307

2 (0,6%)

2

0

0

0

Positive samples in PCR reaction in a Real time mode with the threshold line before 30 cycles, thus indicating the presence of sufficient RNA RNase P gene indicating the specimen is of acceptable quality. At the same time primary infection and 2 passages on a cell culture have been conducted out of them in 3 cases (from materials from Night Heron and 2 Mallards ) cytopathogenic virus effect is detected. These isolates are typed in hemagglutination reaction and hemagglutination inhibition of reaction. Preliminary results of virological researches see below.

Table 3

Results of virological researches

# of lab

Quarter

Name of a bird

Date of sampling

Place of sampling

PCR result

Results of subtyping

I

Black-crowned night heron

22.07.2009

Surroundings of Tokmok town

positive

гр А (Н1)?

1740

VI

Mallard

28.10.2010

Issyk-Kul oblast, Ton district, surroundings of Toguz-Bulak village

positive

гр В/Florida?

1754

VI

Mallard

29.10.2010

Issyk-Kul oblast, Ton district, surroundings of Toguz-Bulak village

positive

гр А (Н3)?



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